Proteomic analysis of ACTN4-interacting proteins reveals its a putative involvement in mRNA metabolism
2010 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 397, no 2, 192-196 p.Article in journal (Refereed) Published
Alpha-actinin 4 (ACTN4) is an actin-binding protein. In the cytoplasm, ACTN4 participates in structural organisation of the cytoskeleton via cross-linking of actin filaments. Nuclear localisation of ACTN4 has also been reported, but no clear role in the nucleus has been established. In this report, we describe the identification of proteins associated with ACTN4 in the nucleus. A combination of two-dimensional gel electrophoresis (2D-GE) and MALDI-TOF mass-spectrometry revealed a large number of ACTN4-bound proteins that are involved in various aspects of mRNA processing and transport. The association of ACTN4 with different ribonucleoproteins suggests that a major function of nuclear ACTN4 may be regulation of mRNA metabolism and signaling.
Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam , 2010. Vol. 397, no 2, 192-196 p.
Alpha-actinin 4; mRNA processing; hnRNP; MALDI-TOF
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-58241DOI: 10.1016/j.bbrc.2010.05.079ISI: 000279718900013OAI: oai:DiVA.org:liu-58241DiVA: diva2:337994