Micromechanical Stimulation of Single Cells Using Polymer Actuators
2010 (English)In: Actuator 2010, MESSE BREMEN-HVG HANSEATISCHE VERANSTALTUNGS-GMBH , 2010, 429-431 p.Conference paper (Refereed)
The effect of mechanical forces on cells is a relatively unexplored area of cell biology. However, mechanical forces play an important role in cell proliferation and function. For instance in muscle contraction, bone growth, and morphogenesis. There is only a limited selection of tools to study this on a single cell level and/or follow the events in real time. Here, we present a new tool in order to mechanically stimulate cells both on a single cell level as well as parts of functional monolayers. The device is designed so that it can be used together with different imaging techniques used in cell biology. The device comprises polypyrrole microactuators. These microactuators can be operated in salt solutions including cell culture media, which makes them well suited for cell biology applications. In addition, polypyrrole is known to be biocompatible, making them a good choice for this device. We will present a device with which we can stretch cells and show the cellular response to this mechanical stimulation. Since the dawn of eukaryotic cells many parallel molecular mechanisms that respond to mechanical stimuli have evolved. This technology allows us to begin the investigation of these mechanisms on a single cell level.
Place, publisher, year, edition, pages
MESSE BREMEN-HVG HANSEATISCHE VERANSTALTUNGS-GMBH , 2010. 429-431 p.
polymer microactuators; mechanostimulation; polypyrrole; MDCK cells
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-58220ISI: 000279861200087ISBN: 978-3-933339-13-3OAI: oai:DiVA.org:liu-58220DiVA: diva2:338064
Actuator 2010, 14-16 June 2010, Bremen, Germany