liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Rapid UHPLC Method for Simultaneous Determination of Vancomycin, Terbinafine, Spironolactone, Furosemide and Their Metabolites: Application to Human Plasma and Urine
Silesian Technical University, Poland.
Silesian Technical University, Poland.
Östergötlands Läns Landsting, Heart Centre, Department of Clinical Physiology.
2010 (English)In: Analytical Sciences, ISSN 0910-6340, Vol. 26, no 7, 755-759 p.Article in journal (Refereed) Published
Abstract [en]

The ultra high performance liquid chromatography (UHPLC)-UV method for the simultaneous determination of furosemide, saluamine (furosemide metabolite), spironolactone, carnenone (spironolactone active metabolite), terbinafine, N-desmethylcarboxy terbinafine (terbinafine metabolite) and vancomycin in human plasma and urine is proposed. Good separation of the analytes was achieved with the gradient RP-UHPLC-UV with the mobile phase composed as acetonitrile and 0.1% formic acid. The determined substances were eluted from a Hypersil GOLD C(18)e (50 mm x 2.1 mm, 1.7 mu m particles) column in 3.3 min. Good linear relationships were observed for all of the analytes (R-2 higher than 0.994). The limit of detection (LOD) values varied from 0.01 to 0.07 mu g ml(-1), with vancomycin as an exception (0.11 mu g ml(-1)). After protein precipitation and solid-phase extraction, samples of plasma and urine were analyzed. Thanks to the short analysis time and small quantities of urine or plasma needed, this method can be applied to routine clinical analysis.

Place, publisher, year, edition, pages
The Japan Society of Analytical Chemistry / The Japan Society for Analytical Chemistry , 2010. Vol. 26, no 7, 755-759 p.
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-58173DOI: 10.2116/analsci.26.755ISI: 000280017000005PubMedID: 20631435OAI: diva2:342912
Available from: 2010-08-11 Created: 2010-08-09 Last updated: 2010-08-23

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Clinical Physiology
In the same journal
Analytical Sciences
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 70 hits
ReferencesLink to record
Permanent link

Direct link