G protein-coupled receptor mediated sensing of TMA
2009 (English)In: PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE, ISSN 1876-6196, Vol. 1, no 1, 321-324 p.Article in journal (Refereed) Published
A new approach for the detection of trimethylamine (TMA) using recombinant Xenopus laevis melanophores was developed. The cells were genetically modified to express the mouse trace amine-associated receptor 5 (mTAAR5), a G protein-coupled receptor from the olfactory epithelium, which conferred high sensitivity to TMA. A focused chemical screen allowed the discovery of additional, previously unknown stimuli of mTAAR5. The cell-based sensor demonstrated no sensitivity to trimethylamine N-oxide (TMAO), making it suitable for a convenient evaluation of TMA levels in fish tissue extracts. The developed gas measurement platform was able to detect TMA from 1 to 100 ppm within thirty-five minutes.
Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2009. Vol. 1, no 1, 321-324 p.
Cell-based sensor; trace amine-associated receptor; TAAR5; trimethylamine detection; Xenopus laevis melanophores
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-58424DOI: 10.1016/j.proche.2009.07.080ISI: 000275995600216OAI: oai:DiVA.org:liu-58424DiVA: diva2:343200