Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose
2010 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 25, no 10, 2205-2211 p.Article in journal (Refereed) Published
In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 mu m diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 mu M). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration.
Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2010. Vol. 25, no 10, 2205-2211 p.
ZnO nanorods; Functionalisation; Intracellular glucose; Electrochemical sensor
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-58381DOI: 10.1016/j.bios.2010.02.025ISI: 000278702600004OAI: oai:DiVA.org:liu-58381DiVA: diva2:343299
Muhammad Asif, Syed Usman Ali, Omer Nour, Magnus Willander, Cecilia Brännmark, Peter Strålfors, Ulrika Englund, Fredrik Elinder and Bengt Danielsson, Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose, 2010, Biosensors & bioelectronics, (25), 10, 2205-2211.
Copyright: Elsevier Science B.V., Amsterdam.