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Biotinylation of ZnO Nanoparticles and Thin Films: A Two-Step Surface Functionalization Study
Linköping University, Department of Physics, Chemistry and Biology, Molecular Surface Physics and Nano Science. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Semiconductor Materials. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Surface Physics and Nano Science. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics. Linköping University, The Institute of Technology.
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2010 (English)In: ACS APPLIED MATERIALS and INTERFACES, ISSN 1944-8244, Vol. 2, no 7, 2128-2135 p.Article in journal (Refereed) Published
Abstract [en]

This study reports ZnO nanoparticles and thin film surface modification using a two-step functionalization strategy. A small silane molecule was used to build up a stabilizing layer and for conjugation of biotin (vitamin B7), as a specific tag. Biotin was chosen because it is a well-studied bioactive molecule with high affinity for avidin. ZnO nanoparticles were synthesized by electrochemical deposition under oxidizing condition, and ZnO films were prepared by plasma-enhanced metal organic chemical vapor deposition. Both ZnO nanoparticles and ZnO thin films were surface modified by forming a (3-mercaptopropyl)trimethoxysilane (MPTS) layer followed by attachment of a biotin derivate. lodoacetyl-PEG2-biotin molecule was coupled to the thiol unit in MPTS through a substitution reaction. Powder X-ray diffraction, transmission electron microscopy, X-ray photoemission electron microscopy, atomic force microscopy. X-ray photoelectron spectroscopy, and near-edge X-ray absorption fine structure spectroscopy were used to investigate the as-synthesized and functionalized ZnO materials. The measurements showed highly crystalline materials in both cases with a ZnO nanoparticle diameter of about 5 nm and a grain size of about 45 nm for the as-grown ZnO thin films. The surface modification process resulted in coupling of silanes and biotin to both the ZnO nanoparticles and ZnO thin films. The two-step functionalization strategy has a high potential for specific targeting in bioimaging probes and for recognition studies in biosensing applications.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA , 2010. Vol. 2, no 7, 2128-2135 p.
Keyword [en]
ZnO nanoparticles, thin films XRD, TEM, XPS, NEXAFS, biotin, PEEM
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-58542DOI: 10.1021/am100374zISI: 000280367000049OAI: oai:DiVA.org:liu-58542DiVA: diva2:343386
Available from: 2010-08-13 Created: 2010-08-13 Last updated: 2015-05-29
In thesis
1. Synthesis, Surface Modification, and Characterization of Metal Oxide Nanoparticles: Nanoprobes for Signal Enhancement in Biomedical Imaging
Open this publication in new window or tab >>Synthesis, Surface Modification, and Characterization of Metal Oxide Nanoparticles: Nanoprobes for Signal Enhancement in Biomedical Imaging
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis we investigate crystalline metal oxide nanoparticles of our own design to obtain nanoprobes for signal enhancement and bioimaging purposes. We report fabrication, surface modification and characterization of nanoparticles based on zinc (Zn), and rare earths (i.e. gadolinium (Gd) and europium (Eu)) singly and in combination. Our ZnO nanoparticles show high potential as fluorescent probes and Gd2O3 nanoparticles are promising as nanoprobes for MR signal enhancement. A combined Zn, Gd material is investigated as a potential dual probe. Interestingly, this nanoprobe shows, compared to the pure oxides, both increased fluorescent quantum yield and do induce improved relaxivity and by that enhanced MR signal. Nanoparticles composed of Eu doped Gd2O3 are also investigated in terms of their ability to interact with silicon surfaces. The presence of nanoparticles shows a catalytic effect on the annealing procedure of SiOx.

Surface modification of Gd and Zn based nanoparticles is performed, in a first step to improve stabilization of the nanoparticle core. Both carboxylic acids (paper I) and a thiol terminated silane (paper II and III) are used for this purpose. In a second step, a polyethylene glycol (PEG) is used for surface modification, to increase the biocompatibility of the nanoparticles. The Mal PEG NHS is chemically linked to thiol terminated silane groups via a maleimide coupling (Paper II). The presence of free NHS functional groups is intended to enable further linking of specific molecules for targeting purposes. The fluorescent dye rhodamine was, as a proof of concept, linked via the NHS functional group to the PEGylated Gd2O3 nanoparticles (Paper II). In Paper III, an alternative linking strategy is investigated, using iodized PEG2-Biotin for coupling via the iodide unit to the thiol terminated silane on ZnO nanoparticles. The resulting surface modified nanoparticles are investigated by means of coordination chemistry and coupling efficiency using X-ray photoelectron spectroscopy, near edge X-ray absorption fine structure  spectroscopy and infrared spectroscopy.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2013. 58 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1510
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-91849 (URN)978-91-7519-646-6 (ISBN)
Public defence
2013-05-24, Planck, Fysikhuset, Campus Valla, Linköpings universitet, Linköping, 10:15 (Swedish)
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Available from: 2013-05-03 Created: 2013-05-03 Last updated: 2015-06-03Bibliographically approved

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Selegård, LinnéaKhranovskyy, VolodymyrSöderlind, FredrikVahlberg, CeciliaAhrén, MariaKäll, Per-OlovYakimova, RositsaUvdal, Kajsa

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Selegård, LinnéaKhranovskyy, VolodymyrSöderlind, FredrikVahlberg, CeciliaAhrén, MariaKäll, Per-OlovYakimova, RositsaUvdal, Kajsa
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