Biotinylation of ZnO Nanoparticles and Thin Films: A Two-Step Surface Functionalization Study
2010 (English)In: ACS APPLIED MATERIALS and INTERFACES, ISSN 1944-8244, Vol. 2, no 7, 2128-2135 p.Article in journal (Refereed) Published
This study reports ZnO nanoparticles and thin film surface modification using a two-step functionalization strategy. A small silane molecule was used to build up a stabilizing layer and for conjugation of biotin (vitamin B7), as a specific tag. Biotin was chosen because it is a well-studied bioactive molecule with high affinity for avidin. ZnO nanoparticles were synthesized by electrochemical deposition under oxidizing condition, and ZnO films were prepared by plasma-enhanced metal organic chemical vapor deposition. Both ZnO nanoparticles and ZnO thin films were surface modified by forming a (3-mercaptopropyl)trimethoxysilane (MPTS) layer followed by attachment of a biotin derivate. lodoacetyl-PEG2-biotin molecule was coupled to the thiol unit in MPTS through a substitution reaction. Powder X-ray diffraction, transmission electron microscopy, X-ray photoemission electron microscopy, atomic force microscopy. X-ray photoelectron spectroscopy, and near-edge X-ray absorption fine structure spectroscopy were used to investigate the as-synthesized and functionalized ZnO materials. The measurements showed highly crystalline materials in both cases with a ZnO nanoparticle diameter of about 5 nm and a grain size of about 45 nm for the as-grown ZnO thin films. The surface modification process resulted in coupling of silanes and biotin to both the ZnO nanoparticles and ZnO thin films. The two-step functionalization strategy has a high potential for specific targeting in bioimaging probes and for recognition studies in biosensing applications.
Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA , 2010. Vol. 2, no 7, 2128-2135 p.
ZnO nanoparticles, thin films XRD, TEM, XPS, NEXAFS, biotin, PEEM
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-58542DOI: 10.1021/am100374zISI: 000280367000049OAI: oai:DiVA.org:liu-58542DiVA: diva2:343386