liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Synergy between DNA polymerases increases polymerase chain reaction inhibitor tolerance in forensic DNA analysis
Department of Applied Microbiology, Lund University, Sweden.
Swedish National Laboratory of Forencis Sciences, Linkoping, Sweden.
National Laboratory of Forensic Sciences, Linkoping, Sweden.
National Laboratory of Forensic Sciences, Linkoping, Sweden.
Show others and affiliations
2010 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 405, 192-200 p.Article in journal (Refereed) Published
Abstract [en]

The success rate of diagnostic polymerase chain reaction (PCR) analysis is lowered by inhibitory substancespresent in the samples. Recently, we showed that tolerance to PCR inhibitors in crime scene salivastains can be improved by replacing the standard DNA polymerase AmpliTaq Gold with alternative DNApolymerase–buffer systems (Hedman et al., BioTechniques 47 (2009) 951–958). Here we show thatblending inhibitor-resistant DNA polymerase–buffer systems further increases the success rate of PCRfor various types of real crime scene samples showing inhibition. For 34 of 42 ‘‘inhibited” crime scenestains, the DNA profile quality was significantly improved using a DNA polymerase blend of ExTaq HotStart and PicoMaxx High Fidelity compared with AmpliTaq Gold. The significance of the results was confirmedby analysis of variance. The blend performed as well as, or better than, the alternative DNA polymerasesused separately for all tested sample types. When used separately, the performance of the DNApolymerases varied depending on the nature of the sample. The superiority of the blend is discussed interms of complementary effects and synergy between the DNA polymerase–buffer systems.

Place, publisher, year, edition, pages
-: Elsevier Inc. , 2010. Vol. 405, 192-200 p.
Keyword [en]
DNA polymerase, DNA polymerase blend, Forensic DNA analysis, PCR inhibition, PCR inhibitors, Synergy
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-58545DOI: 10.1016/j.ab.2010.06.028OAI: oai:DiVA.org:liu-58545DiVA: diva2:343478
Available from: 2010-08-13 Created: 2010-08-13 Last updated: 2017-12-12Bibliographically approved

Open Access in DiVA

fulltext(1091 kB)1195 downloads
File information
File name FULLTEXT02.pdfFile size 1091 kBChecksum SHA-512
f030a18b69d082ed5a1a1231a26bed3a2809492aeb738255dae61237d6bf38c0df3062ab391b1a154e7702ba044a1481a21b8a7a7295ed97430c2ecf9db216cc
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Nordgaard, AndersRasmusson, BirgittaAnsell, Ricky

Search in DiVA

By author/editor
Nordgaard, AndersRasmusson, BirgittaAnsell, Ricky
By organisation
Molecular geneticsThe Institute of Technology
In the same journal
Analytical Biochemistry
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 1195 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 208 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf