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Design and application of software sensors for monitoring of fed-batch cultivations containing recombinant Escherichia coli
Linköping University, Department of Physics, Chemistry and Biology.
2010 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Bioprocesses are industrial processes that use living cells to produce desired products. These processes are complex and require advanced monitoring and control in order to reach their highest potential. Software sensors are a potent tool for real time monitoring and control of bioprocesses. In this project, three novel software sensors were designed and tested in three fed-batch cultivations containing recombinant Escherichia coli producing green fluorescence protein (GFP). Special attention was given to the sensors for reacting to any stress triggered when production of the recombinant protein was initiated by an inducer molecule.

 

The sensors calculated the oxygen consumption per substrate, carbon emission per substrate and the sum of the specific growth rates of various acids. Their mathematical algorithms were derived from already existing software sensors and programmed on to computer software designed for monitoring and control.

 

The results showed that all three sensors gave stable signals as expected. Unfortunately they did not provide any useful information regarding the recombinant protein expression in this particular study. Complications such as measurement errors and incorrect system configurations also created difficulties. It is clear however, that the sensors work well and should be highly considered for further studies.

Place, publisher, year, edition, pages
2010. , 59 p.
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:liu:diva-59301ISRN: LITH-IFM-A-EX--10/2366--SEOAI: oai:DiVA.org:liu-59301DiVA: diva2:351099
Uppsok
Physics, Chemistry, Mathematics
Examiners
Available from: 2010-10-11 Created: 2010-09-13 Last updated: 2010-10-11Bibliographically approved

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Design and application of software sensors for monitoring of fed-batch cultivations containing recombinant Escherichia coli(1090 kB)826 downloads
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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf