liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections
Austrian Academy of Science.
Austrian Academy of Science.
IMP, Vienna.
2007 (English)In: NATURE PROTOCOLS, ISSN 1754-2189, Vol. 2, no 6, 1508-1514 p.Article in journal (Refereed) Published
Abstract [en]

Here we describe a method for sensitive and specific histological detection of microRNAs (miRNAs) by in situ hybridization. The protocol focuses on the use of locked nucleic acids (LNAs), which are bi-cyclic RNA analogs that allow a significant increase in the hybridization temperature and thereby an enhanced stringency for short probes as required for miRNA detection. The protocol is optimized for cryosections in order to study the spatial and temporal expression of miRNAs with high sensitivity and resolution. We detail how to construct probes, set up and conduct an LNA in situ hybridization experiment. In addition, we discuss alternative colorimetric strategies that can be used to effectively detect and visualize miRNAs including double staining with other markers. Setting up and conducting the in situ experiment is estimated to take similar to 1 week, assuming that all the component parts are readily available.

Place, publisher, year, edition, pages
Nature Publishing Group , 2007. Vol. 2, no 6, 1508-1514 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-59694DOI: 10.1038/nprot.2007.153ISI: 000253138900021OAI: oai:DiVA.org:liu-59694DiVA: diva2:353064
Available from: 2010-09-23 Created: 2010-09-23 Last updated: 2010-09-23

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Alenius, Mattias

Search in DiVA

By author/editor
Alenius, Mattias
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 107 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf