liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Monitoring of troponin release from cardiomyocytes during exposure to toxic substances using surface plasmon resonance biosensing
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Chemistry.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
2010 (English)In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY, ISSN 1618-2642, Vol. 398, no 3, 1395-1402 p.Article in journal (Refereed) Published
Abstract [en]

Troponin T (TnT) is a useful biomarker for studying drug-induced toxicity effects on cardiac cells. We describe how a surface plasmon resonance (SPR) biosensor was applied to monitor the release of TnT from active HL-1 cardiomyocytes in vitro when exposed to cardiotoxic substances. Two monoclonal human TnT antibodies were compared in the SPR immunosensor to analyse the TnT release. The detection limit of TnT was determined to be 30 ng/ml in a direct assay set-up and to be 10 ng/ml in a sandwich assay format. Exposure of the cardiomyocytes to doxorubicin, troglitazone, quinidine and cobalt chloride for periods of 6 and 24 h gave significant SPR responses, whereas substances with low toxicity showed insignificant effects (ascorbic acid, methotrexate). The SPR results were verified with a validated immunochemiluminescence method which showed a correlation of r(2)=0.790.

Place, publisher, year, edition, pages
Springer Science Business Media , 2010. Vol. 398, no 3, 1395-1402 p.
Keyword [en]
Surface plasmon resonance, Troponin T, Cardiotoxicity, In vitro toxicity, HL-1 cardiomyocytes, Cytotoxicity screening
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-59731DOI: 10.1007/s00216-010-4041-9ISI: 000281670400026OAI: oai:DiVA.org:liu-59731DiVA: diva2:353130
Note

The original publication is available at www.springerlink.com: Henrik Andersson, Bertil Kågedal and Carl-Fredrik Mandenius, Monitoring of troponin release from cardiomyocytes during exposure to toxic substances using surface plasmon resonance biosensing, 2010, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, (398), 3, 1395-1402. http://dx.doi.org/10.1007/s00216-010-4041-9 Copyright: Springer Science Business Media http://www.springerlink.com/

Available from: 2010-09-24 Created: 2010-09-24 Last updated: 2015-05-29

Open Access in DiVA

fulltext(314 kB)1124 downloads
File information
File name FULLTEXT02.pdfFile size 314 kBChecksum SHA-512
7feb7b5f49888ce4eb190fd41a43b7c0a90da8432d558171356dec2beb1130a8abd8e512e373051e1f8b6e0911ee9b7ef936ec60a68fbb3a08f448f54a570d66
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Andersson, HenrikKågedal, BertilMandenius, Carl-Fredrik

Search in DiVA

By author/editor
Andersson, HenrikKågedal, BertilMandenius, Carl-Fredrik
By organisation
BiotechnologyFaculty of Science & EngineeringClinical ChemistryFaculty of Health SciencesDepartment of Clinical ChemistryThe Institute of Technology
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 1126 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 214 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf