liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Immunohistochemical distribution of cyclic GMP-dependent protein kinase-1 in human prostate tissue
Hannover Medical School.
Hannover Medical School.
Show others and affiliations
2007 (English)In: European Urology, ISSN 0302-2838, E-ISSN 1873-7560, Vol. 52, no 2, 495-501 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES: Phosphodiesterase 5 (PDE5) inhibitors improve smooth muscle relaxation and therefore are considered for pharmacotherapy of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS). Cyclic guanosine monophosphate (cGMP)-dependent protein kinase-1 (cGKI) has been identified as one of the downstream targets for cGMP. The aim of the present study was to evaluate, by means of immunohistochemistry and Western blot analysis, the expression and localization of cGKI isoforms in relation to smooth muscle alpha-actin and cGMP in the human prostate.

METHODS: Cryostat sections of tissue segments excised from the transition zone of human prostates from 11 patients (aged 54-68 yr) were incubated with primary antibodies directed against smooth muscle alpha-actin, cGMP, cGKI, cGKIalpha, and cGKIbeta. Visualization of double-labelled immunofluorescent staining was achieved by laser microscopy. Western blot analysis was performed to confirm the expression of cGKI isoforms.

RESULTS: Immunoreactivities specific for cGKI, cGKIalpha, and cGKIbeta were observed in the smooth musculature of the transition zone. Double-staining revealed the colocalization of smooth muscle alpha-actin, cGMP, and cGKI isoforms in smooth muscle cells of the fibromuscular stroma. The expression of cGKI isoforms was confirmed by Western blot analysis.

CONCLUSIONS: Our results confirm the presence of cGKI isoforms alpha and beta in the transition zone of human prostate tissue. In addition, the colocalization of alpha-actin, cGMP, and cGKI isoforms provides further evidence for a significant role of the nitric oxide/cGMP pathway in the regulation of smooth muscle contractility in human prostate tissue and therefore could provide additional targets for pharmacotherapy of BPH and LUTS.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam , 2007. Vol. 52, no 2, 495-501 p.
Keyword [en]
Benign prostatic hyperplasia; Cyclic nucleotides; Lower urinary tract symptoms; Phosphodiesterase 5; Phosphodiesterase 5 inhibitor; Protein kinase G
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-59804DOI: 10.1016/j.eururo.2007.02.004ISI: 248438900028PubMedID: 17329019OAI: diva2:353329
Available from: 2010-09-25 Created: 2010-09-24 Last updated: 2011-08-17

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hedlund, Petter
By organisation
Clinical PharmacologyFaculty of Health Sciences
In the same journal
European Urology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 29 hits
ReferencesLink to record
Permanent link

Direct link