liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Female pig urethral tone is dependent on Rho guanosine triphosphatases and Rho-associated kinase
University of Lund.
University of Lund.
University of Lund.
University of Lund.
2004 (English)In: Journal of Urology, ISSN 0022-5347, E-ISSN 1527-3792, Vol. 171, no 5, 1955-1958 p.Article in journal (Refereed) Published
Abstract [en]

PURPOSE: Circular smooth muscle of the urethra generates spontaneous myogenic tone of relevance for the maintenance of continence. We tested if Rho guanosine triphosphatases (GTPases) and Rho-associated kinase (ROK) are involved in the generation of urethral tone.

MATERIALS AND METHODS: Small circular strips of female pig urethra were dissected out and mounted for recording isometric force. The effect of pharmacological agents known to modulate the activity of Rho GTPases or ROK was examined. The intracellular calcium concentration was measured using fura-2.

RESULTS: Urethral tone was abolished by removing extracellular calcium or by adding the calcium antagonist felodipine. The decrease in force was closely related to a decrease in intracellular calcium concentration, indicating that tone depends on membrane associated mechanisms. Toxin B, which inactivates Rho GTPases, and Y 27632, which inhibits ROK, completely abolished tone in the female pig urethra. The latter effect occurred without any change in the intracellular calcium concentration.

CONCLUSIONS: The results suggests that urethral tone depends on activity in G-protein coupled pathways and inhibition of this activity is sufficient for urethral tone relaxation. Thus, to our knowledge a new pathway in the generation of urethral tone, which might be acted on by autonomic nerves during micturition, has been identified.

Place, publisher, year, edition, pages
Elsevier Science B.V. Amsterdam , 2004. Vol. 171, no 5, 1955-1958 p.
Keyword [en]
urethra; swine; muscle, smooth; rho-associated kinase; GTP-binding proteins
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-59777DOI: 10.1097/01.ju.0000121601.95857.5aISI: 220872800055PubMedID: 15076320OAI: oai:DiVA.org:liu-59777DiVA: diva2:353374
Available from: 2010-09-26 Created: 2010-09-24 Last updated: 2017-12-12

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Hedlund, Petter

Search in DiVA

By author/editor
Hedlund, Petter
In the same journal
Journal of Urology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 38 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf