The forensic DNA profile index - a tool for comparison of electropherograms
2010 (English)In: English Speaking Working Group (ESWG), International Society of Forensic Genetics (ISFG) Stockholm, Sweden, 2010Conference paper (Other academic)
Assessment of DNA profile quality is vital in forensic DNA analysis, both in order to determine the evidentiary value of DNA results and to compare the performances of different DNA analysis protocols. Generally the quality assessment is performed through manual examination of the DNA profiles based on empirical knowledge, or by comparing the intensities (allelic peak heights) of the capillary electrophoresis electropherograms.
We recently1 developed a ranking index for unbiased and quantitative quality assessment of forensic DNA profiles, the forensic DNA profile index (FI).
Core of presentation
FI uses electropherogram data to combine the intensities of the allelic peaks with the balances within and between loci, using Principal Components Analysis. Here we present the construction of FI. We explain the mathematical and statistical methodologies used and present details about the applied data reduction method. Thereby we show how to adapt the ranking index for any STR-based forensic DNA typing system through validation against a manual grading scale and calibration against a specific set of DNA profiles.
1 Hedman J, Nordgaard A, Rasmusson B, Ansell R, Rådström P (2009), Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modelling of DNA profiles, Biotechniques 47, 351-358.
Place, publisher, year, edition, pages
Probability Theory and Statistics
IdentifiersURN: urn:nbn:se:liu:diva-59853OAI: oai:DiVA.org:liu-59853DiVA: diva2:353586
English Speaking Working Group (ESWG), International Society of Forensic Genetics (ISFG) September 8 – 11, 2010, Stockholm, Sweden