Blood interference in fiber-optical based fluorescence guided resection of glioma using 5-aminolevulinic acid
2011 (English)In: Photonic Therapeutics and Diagnostics VII / [ed] Nikiforos Kollias; Bernard Choi; Haishan Zeng; Hyun Wook Kang; Bodo E. Knudsen; Brian J. Wong; Justus F. R. Ilgner; Kenton W. Gregory; Guillermo J. Tearney; Laura Marcu; Henry Hirschberg; Steen J. Madsen; Andreas Mandelis; Anita Mahadevan-Jansen; E. Duco Jansen, SPIE - International Society for Optical Engineering, 2011, 78833R-1-78833R-10 p.Conference paper (Refereed)
Fluorescence guidance in brain tumor resection is performed intra-operatively where bleeding is included. When using fiber-optical probes, the transmission of light to and from the tissue is totally or partially blocked if a small amount of blood appears in front of the probe. Sometimes even after rinsing with saline, the remnant blood cells on the optical probe head, disturb the measurements. In such a case, the corresponding spectrum cannot be reliably quantified and is therefore discarded. The optimal case would be to calculate and take out the blood effect systematically from the collected signals. However, the first step is to study the pattern of blood interference in the fluorescence spectrum. In this study, a fiber-optical based fluorescence spectroscopy system with a laser excitation light of 405 nm (1.4 J/cm2) was used during fluorescence guided brain tumor resection using 5-aminolevulinic acid (5-ALA). The blood interference pattern in the fluorescence spectrum collected from the brain was studied in two patients. The operation situation was modeled in the laboratory by placing blood drops from the finger tip on the skin of forearm and the data was compared to the brain in vivo measurements. Additionally, a theoretical model was developed to simulate the blood interference pattern on the skin autofluorescence. The blood affects the collected fluorescence intensity and leaves traces of oxy and deoxy-hemoglobin absorption peaks. According to the developed theoretical model, the autofluorescence signal is considered to be totally blocked by an approximately 500 μm thick blood layer.
Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2011. 78833R-1-78833R-10 p.
, Proceedings of SPIE (Progress in biomedical optics and imaging), ISSN 1605-7422 ; Vol. 7883
Fluorescence guided resection (FGR), brain tumor, protoporphyrin IX (PpIX), blood distortion model
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-60151DOI: 10.1117/12.879067ISI: 000293947200061ISBN: 9780819484208OAI: oai:DiVA.org:liu-60151DiVA: diva2:355353
Photonic Therapeutics and Diagnostics VII, 22-24 January 2011, San Francisco, California, USA
Original Publication: Neda Haj-Hosseini, Shannely Lowndes, Göran Salerud and Karin Wårdell, Blood interference in fiber-optical based fluorescence guided resection of glioma using 5-aminolevulinic acid, 2011, SPIE, Photonics West. http://dx.doi.org/10.1117/12.879067 Copyright 2011 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
The publication is also included in the series Progress in Biomedical Optics and Imaging (ISSN 1605-7422) vol. 12 issue 1.2010-10-062010-10-062016-08-31Bibliographically approved