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Silicon based affinity biochips viewed with imaging ellipsometry
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology . Linköping University, The Institute of Technology.
School of Chemical and Physical Sciences, Victoria University, Wellington, New Zealand.
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics . Linköping University, The Institute of Technology.ORCID iD: 0000-0002-2071-7768
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology . Linköping University, The Institute of Technology.
2000 (English)In: Measurement science and technology, ISSN 0957-0233, E-ISSN 1361-6501, Vol. 11, no 6, 801- p.Article in journal (Refereed) Published
Abstract [en]

In this paper we report on the fabrication of an affinity biochip with a matrix of 900 targets for detection with imaging ellipsometry. Two methods of fabrication of chips are shown: one based on wet etching of a silicon surface and the other on the preparation of so-called tension wells on the silicon surface. The dispensing of reagents and ligands was performed using a pipetting robot equipped with a micro-capillary, a syringe pump and micro-stepping motors. Measurements were performed on the chips in real time with carbohydrate model substances selected for six common lectins. Affinity binding was shown for three of the tested model substances.

Place, publisher, year, edition, pages
2000. Vol. 11, no 6, 801- p.
Keyword [en]
affinity biochip, imaging ellipsometry, silicon
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-60385DOI: 10.1088/0957-0233/11/6/325OAI: oai:DiVA.org:liu-60385DiVA: diva2:356527
Available from: 2010-10-12 Created: 2010-10-12 Last updated: 2017-12-12

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Jager, EdwinMandenius, Carl-Fredrik

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