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Evaluating Multi-Lineage Induction of Human Dermal FibroblastsUsing Gene Expression Analysis
Linköping University, Department of Clinical and Experimental Medicine, Plastic Surgery, Hand Surgery and Burns . Linköping University, Faculty of Health Sciences. (Laboratory for Reconstructive Plastic Surgery)
Linköping University, Department of Clinical and Experimental Medicine, Plastic Surgery, Hand Surgery and Burns . Linköping University, Faculty of Health Sciences. (Laboratory for Reconstructive Plastic Surgery)
Linköping University, Department of Clinical and Experimental Medicine, Plastic Surgery, Hand Surgery and Burns . Linköping University, Faculty of Health Sciences. (Laboratory for Reconstructive Plastic Surgery)
Department of Surgery, Section of Plastic Surgery and Burn Centre, Haukeland University Hospital, Bergen, Norway.
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(English)Manuscript (preprint) (Other academic)
Abstract [en]

During the past decades, several adult stem cell populations from a range of tissues have been characterized. Since principally all human cells contain the same genetic material, the specific gene expression profile determines the cell phenotype. The notion of terminally differentiated somatic cells being necessarily restricted to one phenotype has been challenged, and instead an inherent range of plasticity for any given cell type has been suggested. We have in previous work shown that normal human dermal fibroblasts have an inherent plasticity and can be induced to differentiate towards adipogenic, chondrogenic, endotheliogenic and osteogenic lineages when subjected to defined induction media. The aim of the present study was to further study the differentiation of human dermal fibroblasts on a gene expression level. This was achieved by employing genome wide expression analysis using microarray technology. Selected gene expression was also evaluated over time using real-time PCR. Several master regulatory genes important for lineage commitment, as well as phenotypically relevant genes, were found regulated in the respective induced cultures. The results obtained in this study strengthen previously published results showing an inherent ability for controllable phenotype alteration of human dermal fibroblasts in vitro. We conclude that adipogenic, chondrogenic, endotheliogenic and osteogenic induction results in novel phenotypes that show a genetic readiness for lineage-specific biological functionality.

Keyword [en]
Adipogenesis, Chondrogenesis, Endotheliogenesis, Osteogenesis, Fibroblasts, Microarray
National Category
Surgery Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-60877OAI: oai:DiVA.org:liu-60877DiVA: diva2:359629
Available from: 2010-10-28 Created: 2010-10-28 Last updated: 2010-10-29
In thesis
1. Differentiation of Human Dermal Fibroblasts and Applications in Tissue Engineering
Open this publication in new window or tab >>Differentiation of Human Dermal Fibroblasts and Applications in Tissue Engineering
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Tissue engineering applies principles of biology and engineering to the development of functional substitutes for damaged or lost tissues. Tools for the neo-generation of tissue in tissue engineering research include cells, biomaterials and soluble factors.

One main obstacle in tissue engineering is the limited availability of autologous tissue specific progenitor cells. This has led to interest into using autologous cells with stem cell plasticity. Bone marrow derived stem cells were the first adult stem cells shown to have multilineage potential. Since, several reports have been published indicating that cells from other tissues; fat, muscle, connective tissue e.g., possess potential to differentiate into lineages distinct from their tissue of origin.

The optimal cell type for use in tissue engineering applications should be easy to obtain, cultivate and store. The human dermal fibroblast is an easily accessible cell source, which after routine cell expansion gives a substantial cell yield from a small skin biopsy. Hence, the dermal fibroblast could be a suitable cell source for tissue engineering applications.The main aim of this thesis was to investigate the differentiation capacity of human dermal fibroblasts, and their possible applications in bone and cartilage tissue engineering applications.

Human dermal fibroblasts were shown to differentiate towards adipogenic, chondrogenic, and osteogenic phenotypes upon subjection to specific induction media. Differentiation was seen both in unrefined primary cultures and in clonal populations (paper I). Fibroblasts could be used to create three-dimensional cartilage- and bone like tissue when grown in vitro on gelatin microcarriers in combination with platelet rich plasma (paper II). 4 weeks after in vivo implantation of osteogenic induced fibroblasts into a fracture model in athymic rats, dense cell clusters and viable human cells were found in the gaps, but no visible healing of defects as determined by CT-scanning (paper III). After the induction towards adipogenic, chondrogenic, endotheliogenic and osteogenic lineages, gene expression analysis by microarray and quantitative real-time-PCR found several master regulatory genes important for lineage commitment, as well as phenotypically relevant genes regulated as compared to reference cultures (paper IV).

In conclusion, results obtained in this thesis suggest an inherent ability for controllable phenotype alteration of human dermal fibroblasts in vitro. We conclude that dermal fibroblasts could be induced towards adipogenic, chondrogenic, endotheliogenic or osteogenic novel phenotypes which suggest a genetic readiness of differentiated fibroblasts for lineage-specific biological functionality, indicating that human dermal fibroblasts might be a suitable cell source in tissue engineering applications.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2010. 62 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1202
Keyword
Tissue Engineering, Human Dermal Fibroblasts, Differentiation, Adipogenesis, Chondrogenesis, Endotheliogenesis, Osteogenesis
National Category
Surgery
Identifiers
urn:nbn:se:liu:diva-60879 (URN)978-91-7393-326-1 (ISBN)
Public defence
2010-11-19, Berzeliussalen, Hälsouniversitetet, Campus US, Linköpings universitet, Linköping, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2010-10-29 Created: 2010-10-28 Last updated: 2010-10-29Bibliographically approved

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Rakar, JonathanJunker, JohanKratz, Gunnar

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Plastic Surgery, Hand Surgery and Burns Faculty of Health SciencesDepartment of Plastic Surgery, Hand surgery UHL
SurgeryCell and Molecular Biology

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