Functionalized ZnO nanorod-based selective magnesium ion sensor for intracellular measurements
2010 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 26, no 3, 1118-1123 p.Article in journal (Refereed) Published
ZnO nanorods were grown on a silver-coated tip of a borosilicate glass capillary (0.7 mu m in tip diameter) and used as selective potentiometric sensor of intracellular free Mg2+. To functionalize the ZnO nanorods for selectivity of Mg2+, a polymeric membrane with Mg2+-selective ionophores were coated on the surface of the ZnO nanorods. These functionalized ZnO nanorods exhibited a Mg2+-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode within the concentration range from 500 nM to 100 mM. Two types of cells, human adipocytes and frog oocytes, were used for the intracellular Mg2+ measurements. The intracellular concentration of free Mg2+ in human adipocytes and frog oocytes were 0.4-0.5 and 0.8-0.9 mM, respectively. Such type of nanoelectrode device paves the way to enable analytical measurements in single living cells and to sense other bio-chemical species at the intracellular level.
Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2010. Vol. 26, no 3, 1118-1123 p.
ZnO nanorods, Potentiometric, Mg2+-selective membrane, Human adipocyte, Frog oocyte
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-62158DOI: 10.1016/j.bios.2010.08.017ISI: 000283813600027OAI: oai:DiVA.org:liu-62158DiVA: diva2:371269
Muhammad Asif, Syed Usman Ali, Omer Nour, Magnus Willander, Ulrika Englund and Fredrik Elinder, Functionalized ZnO nanorod-based selective magnesium ion sensor for intracellular measurements, 2010, BIOSENSORS and BIOELECTRONICS, (26), 3, 1118-1123.
Copyright: Elsevier Science B.V., Amsterdam.