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Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering.
Cellartis AB, Gothenburg, Sweden .
University of Gothenburg.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
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2010 (English)In: JOURNAL OF BIOTECHNOLOGY, ISSN 0168-1656, Vol. 150, no 1, 175-181 p.Article in journal (Refereed) Published
Abstract [en]

Human embryonic stem cell (hESC) derived cardiomyocytes are in the present study being used for testing drug-induced cardiotoxicity in a biosensor set-up. The design of an in vitro testing alternative provides a novel opportunity to surpass previous methods based on rodent cells or cell lines due to its significantly higher toxicological relevance. In this report we demonstrate how hESC-derived cardiomyocytes release detectable levels of two clinically decisive cardiac biomarkers, cardiac troponin T and fatty acid binding protein 3, when the cardiac cells are exposed to the well-known cardioactive drug compound. doxorubicin. The release is monitored by the immuno-biosensor technique surface plasmon resonance, particularly appropriate due to its capacity for parallel and high-throughput analysis in complex media.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2010. Vol. 150, no 1, 175-181 p.
Keyword [en]
In vitro toxicity testing, hESC-derived cardiomyocytes, Doxorubicin, Biosensors, Surface plasmon resonance. SPR
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-62153DOI: 10.1016/j.jbiotec.2010.06.023ISI: 000283693600025OAI: diva2:371280
Available from: 2010-11-19 Created: 2010-11-19 Last updated: 2015-05-29

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Andersson, HenrikDahlenborg, KerstinKågedal, BertilMandenius, Carl-Fredrik
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