Lipid bilayer-induced secondary structure in de novo designed peptides – small changes in primary structure leads to α-helices or β-sheets
(English)Manuscript (Other academic)
We show that it is possible to induce defined secondary structure in de novo designed peptides upon adsorption to negatively charged vesicles and silica nanoparticles, and that the structure is highly dependent on which surface is used, the surface charge, pH of the solution, and above all, peptide primary structure. Results from measurements with far-UV CD demonstrate that the induction of structure induced by the interaction with silica nanoparticles is solely helical, whereas vesicles induce helices or b-sheets, and the difference therein is dependent on small changes in peptide primary structure. Peptide adsorption onto the vesicles was monitored with dynamic light scattering, and it is also shown that the stability of the vesicles is disturbed by an increasing concentration of the arginine-rich peptides, eventually leading to aggregation of the vesicles.
IdentifiersURN: urn:nbn:se:liu:diva-15017OAI: oai:DiVA.org:liu-15017DiVA: diva2:37623