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Anomalous settlement behavior of Ulva linza zoospores on cationic oligopeptide surfaces
Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-1639-5735
Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics. Linköping University, The Institute of Technology.
University of Birmingham.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
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2008 (English)In: Biofouling (Print), ISSN 0892-7014, E-ISSN 1029-2454, Vol. 24, no 4, 303-312 p.Article in journal (Refereed) Published
Abstract [en]

Identification of settlement cues for marine fouling organisms opens up new strategies and methods for biofouling prevention, and enables the development of more effective antifouling materials. To this end, the settlement behaviour of zoospores of the green alga Ulva linza onto cationic oligopeptide self-assembled monolayers (SAMs) has been investigated. The spores interact strongly with lysine- and arginine-rich SAMs, and their settlement appears to be stimulated by these surfaces. Of particular interest is an arginine-rich oligopeptide, which is effective in attracting spores to the surface, but in a way which leaves a large fraction of the settled spores attached to the surface in an anomalous fashion. These 'pseudo-settled' spores are relatively easily detached from the surface and do not undergo the full range of cellular responses associated with normal commitment to settlement. This is a hitherto undocumented mode of settlement, and surface dilution of the arginine-rich peptide with a neutral triglycine peptide demonstrates that both normal and anomalous settlement is proportional to the surface density of the arginine-rich peptide. The settlement experiments are complemented with physical studies of the oligopeptide SAMs, before and after extended immersion in artificial seawater, using infrared spectroscopy, null ellipsometry and contact angle measurements.

Place, publisher, year, edition, pages
2008. Vol. 24, no 4, 303-312 p.
Keyword [en]
Fouling, algae, self-assembled monolayers, cationic peptides, Ulva, spore
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-15018DOI: 10.1080/08927010802192650OAI: oai:DiVA.org:liu-15018DiVA: diva2:37624
Available from: 2008-10-09 Created: 2008-10-09 Last updated: 2017-12-11
In thesis
1. Structural and Functional Studies of De Novo Designed Peptides at Surfaces
Open this publication in new window or tab >>Structural and Functional Studies of De Novo Designed Peptides at Surfaces
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The work presented in this thesis deals with the structural and functional properties of peptides at surfaces. The interaction of peptides with surfaces is an ever so common occurrence in our every day life, from the bug squashed on the windshield of our car to the barnacle on our boat, and from the blood plasma used in the hospital to the proteins in our cells. The effect these occurrences has on our lives is diverse, the bug is annoying whereas the barnacle settlement of ship hull is costly for marine transportation, the blood plasma contains components of vital importance for our immunological defense system and the proteins in our cells are crucial for regulatory processes and life.One part of this thesis, performed as a part of the EU-founded project AMBIO, deals with the concept of marine biofouling. A number of short peptides have been designed, synthesized, and used to investigate their effect on the settlement on marine biofoulers, such as the Ulva linza algae and the Navicula diatom, on template surfaces coated with thin layers of these molecules. The surfaces have been thoroughly investigated with respect of their physio-chemical properties before and after submersion in artificial seawater and ultimately in suspensions containing the organisms. The most interesting results were obtained with an arginine-rich peptide coating that when introduced to Ulva linza zoospores, displayed extensive settlement, compared to reference surfaces. In addition, a large fraction of the settled spores had an abnormal morphology.The other part of this thesis is focused on designed peptides that when adsorbed on a negatively charged surface adopts a well-defined secondary structure, either α-helical or β-sheet. Precisely placed amino acids in the peptides will strongly disfavor structure in solution, primarily due to electrostatic repulsion, but when the peptides are adsorbed on the negatively charged surfaces, they adopt a well-defined secondary structure due to ion pair bonding. These interactions have been thoroughly investigated by systematic variations of the side-chains. In order to determine the factors contributing to the induced structure, several peptides with different amino acid sequences have been synthesized. Factors that have been investigated include 1) the positive charge density, 2) distribution of positive charges, 3) negative charge density, 4) increasing hydrophobicity, and 5) incorporating amino acids with different helix propensities. Moreover, pH dependence and the effect of different interaction partners have also been investigated. It has also been shown that the system can be modified to incorporate a catalytic site that is only active when the helix is formed. This research will increase our understanding of peptide-surface interactions and might be of importance for both bionanotechnology and medicine.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2008. 52 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1199
Keyword
Biofouling, vesicles, nanoparticles, peptide, peptide design, circular dichroism
National Category
Physical Chemistry
Identifiers
urn:nbn:se:liu:diva-15022 (URN)978-91-7393-840-2 (ISBN)
Public defence
2008-09-05, Planck, Campus Valla, Linköpings universitet, Linköping, 10:15 (English)
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Supervisors
Available from: 2008-10-09 Created: 2008-10-09 Last updated: 2017-01-11Bibliographically approved

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Ederth, ThomasNygren, PatrikDu, Chun-XiaBroo, KlasLiedberg, Bo

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