Assessment of blood flow changes in human skin by microdialysis urea clearance
2011 (English)In: Microcirculation, ISSN 1073-9688, Vol. 18, no 3, 198-204 p.Article, review/survey (Refereed) Published
Objective: The aim of this study was to evaluate the urea clearance technique for the measurement of drug-induced blood flow changes in human skin, and compare it with two non-invasive techniques: polarization light spectroscopy and laser Doppler perfusion imaging.
Methods: Fifteen microdialysis catheters were placed intracutaneously on the volar aspect of the forearms of healthy human subjects, and were perfused with nitroglycerine, noradrenaline, and again nitroglycerine, to induce local tissue hyperaemia, hypoperfusion, and hyperaemia, respectively.
Results: Urea clearance, but not the other techniques, detected the changes in blood flow during all three periods of altered flow. The last hyperaemic response was detected by all three methods.
Conclusion: Urea clearance can be used as a relatively simple method to estimate blood flow changes during microdialysis of vasoactive substances, in particular when the tissue is preconditioned in order to enhance the contrast between baseline and the responses to the provocations. Our results support that, in the model described, urea clearance was superior to the optical methods as it detected both the increases and decrease in blood flow, and the returns to baseline between these periods.
Place, publisher, year, edition, pages
Wiley , 2011. Vol. 18, no 3, 198-204 p.
microcirculation; laser Doppler perfusion imaging; polarisation light spectroscopy; ischaemia; reperfusion; hypoperfusion; hyperaemia
IdentifiersURN: urn:nbn:se:liu:diva-63115DOI: 10.1111/j.1549-8719.2010.00077.xISI: 000288759900004OAI: oai:DiVA.org:liu-63115DiVA: diva2:376551
The original title of article IV was "Assessment of blood flow changes in a new pharmacological model of microdosing in human skin by microdialysis urea clearance".2010-12-112010-12-112012-03-21Bibliographically approved