Platelet adhesion to proteins in microplates: from experimental research to clinical evaluation of platelet function
2009 (English)Conference paper (Refereed)
Available platelet function assays differ with respect to the response they measure as well as in applicability, which range from experimental research to clinical evaluation of platelet function. This study focuses on the measurement of static adhesion of platelets in plasma to proteins in microplates. The aim was to describe fundamental adhesive events occurring in the assay and to investigate the ability to detect effects of factors acting both in vitro and in vivo. This communication combines several studies and presents novel interpretations. First of all, in vitro studies showed that platelets adhered to collagen via integrin alpha2beta1 whereas adhesion to surfaces coated with fibrinogen or albumin were dependent on alphaIIbbeta3. Elevated platelet adhesion, dependent on in vitro effects, was detected after addition of known platelet activators. The sensitivity of the assay is illustrated by significantly increased adhesion induced by 30 nmol/L epinephrine. Further in vitro studies showed that inhibition of autocrine activation decreased platelet adhesion. Also, adhesion was synergistically increased when combining two platelet activators at subthreshold levels. The detection of in vivo effects is illustrated by increased platelet adhesion for patients with the thrombosis prone disease essential thrombocythemia compared to healthy controls. The adhesion assay was reproducible in controls over time denoting that the assay can monitor platelet function. Decreased platelet adhesion was observed in vitro after addition of known platelet inhibitors and ex vivo after treatment with clopidogrel alone or in combination with acetylsalicylic acid in patients with a recent acute coronary syndrome. In conclusion, our studies show that this simple and flexible in vitro assay is able to detect elevated as well as decreased platelet adhesion both in vitro and ex vivo.
Place, publisher, year, edition, pages
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-63262OAI: oai:DiVA.org:liu-63262DiVA: diva2:377343
Congress of the International Society on Thrombosis and Haemostasis