Quantitative Analysis of Bone Tissue Engineering Scaffolds and Skull Bones by means of Synchrotron and Conventional X-ray Computed Microtomography
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
The study of internal structure of materials has always been an essential issue in a variety of application fields, from the medical radiology to the materials science. X-ray computed microtomography (with both conventional and synchrotron radiation sources) has a great potential for these purposes because its three-dimensional and non destructive nature as well as the fact that it does not require any sample preparation and it allows to study samples under stress or after consecutive treatments.
The recent developments of new X-ray sources with innovative imaging techniques, as well as novel high resolution detectors, allow moving forward the maximum achievable resolution of this technique to a few micrometers or even less. This contributed to increase its application in biomedical purposes, but also to raise the need for quantitative analysis of the reconstructed data.
Indeed in most of the cases a quantitative characterization of the samples microstructures is needed to better understand their physical and chemical behavior, the effects of manufacturing process or the response to stress. Dedicated software packages have been developed to perform a geometrical and morphological characterization of the samples texture and to evaluate some typical parameters commonly used to classify porous media such as porosity, cell size distribution, connectivity and anisotropy.
In this work two case studies have been considered for the application of a quantitative analysis approach to microtomography datasets: the first concerns the characterization of bone ingrowth within tissue engineering scaffolds, while the second is related to the extraction of morphological descriptors for the architecture of human skull bones.
It will be shown how suitable image processing and analysis techniques are able to effectively quantify significant parameters such as the trabecular thickness of the skull bones as well as the porosity and the degree of connectivity of bone tissue engineering scaffolds. Similar quantitative analysis methods applied to microtomography images have to be considered as an effective methodology for a comprehensive characterization of other biomedical samples.
Place, publisher, year, edition, pages
2010. , 124 p.
X-ray Computed Microtomography, µ-CT, Pore3D, Hydroxyapatite, Bone Scaffolds, Bioglass ceramic scaffolds, Bioactivity, Bioresorption
Other Industrial Biotechnology
IdentifiersURN: urn:nbn:se:liu:diva-63196ISRN: LITH-IFM-A-EX--10/2367--SEOAI: oai:DiVA.org:liu-63196DiVA: diva2:380221
2010-09-17, Planck, Fysikhuset, Linköpings universitet, SE-581 83, LINKÖPING, 10:15 (English)
UppsokPhysics, Chemistry, Mathematics
Tromba, Giuliana, Dr.Brun, Francesco, Dr.
Uvdal, Kajsa, Professor