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Potentiometric cholesterol biosensor based on ZnO nanorods chemically grown on Ag wire
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
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2010 (English)In: Thin Solid Films, ISSN 0040-6090, E-ISSN 1879-2731, Vol. 519, no 3, 1106-1109 p.Article in journal (Refereed) Published
Abstract [en]

An electrochemical biosensor based on ZnO nanorods for potentiometric cholesterol determination is proposed. Hexagon-shaped ZnO nanorods were directly grown on a silver wire having a diameter of 250 mu m using low temperature aqueous chemical approach that produced ZnO nanorods with a diameter of 125250 nm and a length of similar to 1 mu m. Cholesterol oxidase (ChOx) was immobilized by a physical adsorption method onto ZnO nanorods. The electrochemical response of the ChOx/ZnO/Ag biosensor against a standard reference electrode (Ag/AgCl) was investigated as a logarithmic function of the cholesterol concentration (1 x 10(-6)M to 1 x 10(-2)M) showing good linearity with a sensitivity of 35.2 mV per decade and the stable output signal was attained at around 10 s.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2010. Vol. 519, no 3, 1106-1109 p.
Keyword [en]
Potentiometric biosensor; Cholesterol; Cholesterol oxidase; Electrostatic immobilization; ZnO nanorods
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-63930DOI: 10.1016/j.tsf.2010.08.052ISI: 000285075300032OAI: oai:DiVA.org:liu-63930DiVA: diva2:385002
Available from: 2011-01-11 Created: 2011-01-10 Last updated: 2017-12-11
In thesis
1. Chemical fabrication of ZnO nanostructures and their emission properties: Cholesterol biosensing applications utilizing ZnO and Graphene
Open this publication in new window or tab >>Chemical fabrication of ZnO nanostructures and their emission properties: Cholesterol biosensing applications utilizing ZnO and Graphene
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Zinc oxide (ZnO) is an inorganic compound, owing to wide band gap and large binding energy, and holds promising potential in the fields of semiconducting as well as piezoelectric applications with excellent stability and reliability. In addition, ZnO has a plenteous number of nanoscale structures containing unique physical, chemical, electrical, sensing and optical properties. These properties of nanostructures are being unrevealed extensively since last two decades and have become a prominent field of research in nanoscience and nanotechnology.

More specifically, the present dissertation deals with the low temperature synthesis of ZnO nanostructures (nanorods, nanotubes, nanodisks and nanowalls) on a variety of substrates such as silicon, gallium nitride, zinc foil, silver and aluminum; structural characterization and study of their luminescence properties. In paper 1 we investigated the synthesis mechanism of chemically fashioned ZnO nanotubes and their superior emission capability compared to ZnO nanorods with significant enhancements in ultraviolet and visible regions has been studied. These chemically synthesized ZnO nanotubes are further utilized to fabricate a heterostructure with p-GaN thin film in order to achieve white emission (Paper 2). The aim of Paper 3 is to understand the synthesis of ZnO nanorods and their transition into ZnO nanodisks at 55 °C along with temperature dependent micro-photoluminescence studies. However, the second half of the dissertation is devoted to the fabrication of potentiometric cholesterol biosensors through the conjugation of ZnO nanostructures and graphene nanosheets with a thin film of cholesterol oxidase. Paper 4 contains the fabrication of cholesterol biosensor by the deposition of ZnO nanorods on thin silver wire followed by their functionalization under the physical adsorption method. The specificity, reproducibility and stability of the biosensor have been investigated with good linearity slope curve of ~35 mV/ decade. The purpose of papers 5 and 6 is to enhance the sensitivity of the cholesterol biosensor by using ZnO nanowalls and graphene nanosheets as a matrix where the sensitivity of the slope curve is achieved as ~53 and ~82 mV/ decade, respectively.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2011. 67 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1387
Keyword
Zinc oxide, aqueous chemical synthesis, nanostructures, light emitting diode, electrochemical biosensor.
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-71323 (URN)978-91-7393-101-4 (ISBN)
Public defence
2011-09-23, Campus Valla, Linköpings universitet, Linköping, 10:15 (English)
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Available from: 2011-10-11 Created: 2011-10-11 Last updated: 2014-01-15Bibliographically approved

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Qadir Israr, MuhammedRana Sadaf, JamilAsif, MuhammadNour, OmerWillander, Magnus

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