liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Fungicidal activity of human lactoferrin-derived peptides based on the antimicrobial alpha beta region
Gothenburg University.
Uppsala University.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Gothenburg University.
2011 (English)In: INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, ISSN 0924-8579, Vol. 37, no 1, 51-57 p.Article in journal (Refereed) Published
Abstract [en]

Owing to the increasing number of infections in hospitalised patients caused by resistant strains of fungi, there is a need to develop new therapeutic agents for these infections. Naturally occurring antimicrobial peptides may constitute models for developing such agents. A modified peptide sequence (CFQWKRAM-RKVR; HLopt2) based on amino acid residues 20-31 of the N-terminal end of human lactoferrin (hLF) as well as a double-sized human lactoferricin-like peptide (amino acid residues 16-40; HLBD1) were investigated for their antifungal activities in vitro and in vivo. By in vitro assay, HLopt2 was fungicidal at concentrations of 12.5-25 mu g/mL against Cryptococcus neoformans, Candida albicans, Candida krusei, Candida kefyr and Candida parapsilosis, but not against Candida glabrata. HLopt2 was demonstrated to have andgt;= 16-fold greater killing activity than HLBD1. By inducing some helical formation caused by lactam bridges or by extending the assay time (from 2 h to 20 h), HLBD1 became almost comparable with HLopt2 in its fungicidal activity. Killing of C. albicans yeast cells by HLopt2 was rapid and was accompanied by cytoplasmic and mitochondrial membrane permeabilisation as well as formation of deep pits on the yeast cell surface. In a murine C. albicans skin infection model, atopic treatment with the peptides resulted in significantly reduced yields of Candida from the infected skin areas. The antifungal activities of HLopt2 in vitro and in vivo suggest possible potential as a therapeutic agent against most Candida spp. and C. neoformans. The greatly improved antifungal effect of the lactam-modified HLBD1 indicates the importance of amphipathic helix formation for lethal activity.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2011. Vol. 37, no 1, 51-57 p.
Keyword [en]
Fungicidal activity, Peptide, Lactoferricin, Candida albicans
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-64386DOI: 10.1016/j.ijantimicag.2010.08.020ISI: 000285332700010OAI: diva2:390135
Available from: 2011-01-21 Created: 2011-01-21 Last updated: 2011-01-21

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Baltzer, LarsDolphin, Gunnar
By organisation
Department of Physics, Chemistry and BiologyThe Institute of Technology
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 51 hits
ReferencesLink to record
Permanent link

Direct link