Resequencing of positional candidates identifies low frequency IL23R coding variants protecting against inflammatory bowel disease
2011 (English)In: NATURE GENETICS, ISSN 1061-4036, Vol. 43, no 1, 43-U58 p.Article in journal (Refereed) Published
Genome-wide association studies (GWAS) have identified dozens of risk loci for many complex disorders, including Crohns disease(1,2). However, common disease-associated SNPs explain at most similar to 20% of the genetic variance for Crohns disease. Several factors may account for this unexplained heritability(3-5), including rare risk variants not adequately tagged thus far in GWAS(6-8). That rare susceptibility variants indeed contribute to variation in multifactorial phenotypes has been demonstrated for colorectal cancer(9), plasma high-density lipoprotein cholesterol levels(10), blood pressure(11), type 1 diabetes(12), hypertriglyceridemia(13) and, in the case of Crohns disease, for NOD2 (refs. 14,15). Here we describe the use of high-throughput resequencing of DNA pools to search for rare coding variants influencing susceptibility to Crohns disease in 63 GWAS-identified positional candidate genes. We identify low frequency coding variants conferring protection against inflammatory bowel disease in IL23R, but we conclude that rare coding variants in positional candidates do not make a large contribution to inherited predisposition to Crohns disease.
Place, publisher, year, edition, pages
Nature Publishing Group , 2011. Vol. 43, no 1, 43-U58 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-64757DOI: 10.1038/ng.733ISI: 000285683500013OAI: oai:DiVA.org:liu-64757DiVA: diva2:394859