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Detection of pesticide by polymeric enzyme electrodes
University Calcutta, Department Polymer Science and Technology, Calcutta 700009, W Bengal, India.
University Calcutta, Department Polymer Science and Technology, Calcutta .
Jadavpur University, Department Chemistry Engn, Calcutta 700032, India.
Cranfield University, Cranfield Hlth, Silsoe MK45 4DT, Beds, England.
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2008 (English)In: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 69, no 3, 556-561 p.Article in journal (Refereed) Published
Abstract [en]

Screen-printed electrodes (SPEs) containing immobilized acetylcholine esterase (AChE) enzyme were used for the electrochemical determination of organophosphorous (OP) and carbamate pesticides. The extent of AChE deactivation by the pesticide was determined in the presence of acetylcholine (AChCl) substrate. The unique nature of this approach lies in the enzyme immobilization procedure in which AChE was attached to the SPE by in situ bulk polymerization of acrylamide to ensure efficient adherence within the membrane with minimal losses in enzyme activity. Responses were observed for the pesticides Monocrotophos, Malathion, Metasystox and Lannate over the concentration range 0-10 ppb (mu g L-1). (C) 2007 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam , 2008. Vol. 69, no 3, 556-561 p.
Keyword [en]
microencapsulation; pesticide; polyacrylamide; potentiometry; screen-printed electrode; biosensor
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-65172DOI: 10.1016/j.ecoenv.2007.01.004ISI: 000254728800027OAI: diva2:394906
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2013-10-04

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