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Electrochemical immunoassay for free prostate specific antigen (f-PSA) using magnetic beads
University Calcutta, Department Polymer Science and Technology, Calcutta 700009, India.
University Calcutta, Department Polymer Science and Technology, Calcutta 700009, India.
University Calcutta, Department Polymer Science and Technology, Calcutta 700009, India.
Cranfield University, Bedford MK45 4DT, England.
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2008 (English)In: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 20, no 13, 1414-1420 p.Article in journal (Refereed) Published
Abstract [en]

Prostate specific antigen (PSA) is a prominent marker for the prostate carcinoma. It is found in human blood in free (f-PSA) and complex forms. These two forms together are called total PSA (t-PSA). Estimation of both forms is essential to predict malignancy. In this study we report a unique and effective technique of electrochemical detection of f-PSA using magnetic beads on a three-electrode screen-printed sensor. A magnetic bead enzyme linked immunosorbent assay (ELISA) was performed in a cuvette. Following the immunoassay, magnetic beads were recovered by a magnetic concentrator and transferred on the working electrode of the 3-electrode assembly. The amperometric response, a measure of the amount of residual enzyme activity on the beads and hence the concentration of analyte in solution, was determined by addition of enzyme substrate. The device has a detection limit of less than0.1 ng mL(-1) f-PSA and a linear range of 0 to 1 ng mL(-1) f-PSA.

Place, publisher, year, edition, pages
John Wiley andamp;amp; Sons, Ltd , 2008. Vol. 20, no 13, 1414-1420 p.
Keyword [en]
bioanalytical methods; bioassays; biosensors; immunoassays; ELISA
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65168DOI: 10.1002/elan.200804194ISI: 000257618300004OAI: oai:DiVA.org:liu-65168DiVA: diva2:394913
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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P. F. Turner, A.

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CiteExportLink to record
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