Electrochemical immunoassay for free prostate specific antigen (f-PSA) using magnetic beads
2008 (English)In: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 20, no 13, 1414-1420 p.Article in journal (Refereed) Published
Prostate specific antigen (PSA) is a prominent marker for the prostate carcinoma. It is found in human blood in free (f-PSA) and complex forms. These two forms together are called total PSA (t-PSA). Estimation of both forms is essential to predict malignancy. In this study we report a unique and effective technique of electrochemical detection of f-PSA using magnetic beads on a three-electrode screen-printed sensor. A magnetic bead enzyme linked immunosorbent assay (ELISA) was performed in a cuvette. Following the immunoassay, magnetic beads were recovered by a magnetic concentrator and transferred on the working electrode of the 3-electrode assembly. The amperometric response, a measure of the amount of residual enzyme activity on the beads and hence the concentration of analyte in solution, was determined by addition of enzyme substrate. The device has a detection limit of less than0.1 ng mL(-1) f-PSA and a linear range of 0 to 1 ng mL(-1) f-PSA.
Place, publisher, year, edition, pages
John Wiley andamp;amp; Sons, Ltd , 2008. Vol. 20, no 13, 1414-1420 p.
bioanalytical methods; bioassays; biosensors; immunoassays; ELISA
National CategoryEngineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-65168DOI: 10.1002/elan.200804194ISI: 000257618300004OAI: oai:DiVA.org:liu-65168DiVA: diva2:394913