LACTATE, GLUTAMATE AND GLUTAMINE BIOSENSORS BASED ON RHODINISED CARBON ELECTRODES
1994 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 295, no 3, 243-251 p.Article in journal (Refereed) Published
Amperometric enzyme sensors for lactate, glutamate and glutamine were constructed using rhodinised carbon electrodes. All three sensors operated at a potential of +400 mV (Ag/AgCl). Lactate sensors were constructed using lactate oxidase immobilized in hydroxyethylcellulose, overlaid with a cellulose acetate membrane. Using a flow-injection system, the sensors had a linear range of 0.1 to 1.5 mM. Measurements of lactate concentrations from a mammalian cell culture were compared with results obtained from a commercial instrument. A correlation coefficient of r=0.982 (n = 15) was obtained. Glutamate and glutamine sensors were fabricated based on glutaraldehyde immobilization; the former by incorporating glutamate oxidase and the latter based on glutamate oxidase and glutaminase (both had a linear range of 0.1 to 1.5 mM). The feasibility of using these sensors, in conjunction with a previously described glucose sensor, for mammalian cell culture monitoring is discussed
Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 1994. Vol. 295, no 3, 243-251 p.
BIOSENSORS; LACTATE; GLUTAMATE; GLUTAMINE; RHODINISED CARBON ELECTRODES
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-65287ISI: A1994PH14600003OAI: oai:DiVA.org:liu-65287DiVA: diva2:394946