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LACTATE, GLUTAMATE AND GLUTAMINE BIOSENSORS BASED ON RHODINISED CARBON ELECTRODES
CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
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1994 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 295, no 3, 243-251 p.Article in journal (Refereed) Published
Abstract [en]

Amperometric enzyme sensors for lactate, glutamate and glutamine were constructed using rhodinised carbon electrodes. All three sensors operated at a potential of +400 mV (Ag/AgCl). Lactate sensors were constructed using lactate oxidase immobilized in hydroxyethylcellulose, overlaid with a cellulose acetate membrane. Using a flow-injection system, the sensors had a linear range of 0.1 to 1.5 mM. Measurements of lactate concentrations from a mammalian cell culture were compared with results obtained from a commercial instrument. A correlation coefficient of r=0.982 (n = 15) was obtained. Glutamate and glutamine sensors were fabricated based on glutaraldehyde immobilization; the former by incorporating glutamate oxidase and the latter based on glutamate oxidase and glutaminase (both had a linear range of 0.1 to 1.5 mM). The feasibility of using these sensors, in conjunction with a previously described glucose sensor, for mammalian cell culture monitoring is discussed

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 1994. Vol. 295, no 3, 243-251 p.
Keyword [en]
BIOSENSORS; LACTATE; GLUTAMATE; GLUTAMINE; RHODINISED CARBON ELECTRODES
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65287ISI: A1994PH14600003OAI: oai:DiVA.org:liu-65287DiVA: diva2:394946
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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