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AMPEROMETRIC DETECTION OF HISTAMINE AT A QUINOPROTEIN DEHYDROGENASE ENZYME ELECTRODE
CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; UNIV MISSISSIPPI,MED CTR,DEPT BIOCHEM,JACKSON,MS 39216; .
CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; UNIV MISSISSIPPI,MED CTR,DEPT BIOCHEM,JACKSON,MS 39216; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; UNIV MISSISSIPPI,MED CTR,DEPT BIOCHEM,JACKSON,MS 39216; .
1995 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 10, no 07-jun, 569-576 p.Article in journal (Refereed) Published
Abstract [en]

Methylamine dehydrogenase, a tryptophan tryptophyl quinone (TTQ) containing quinoprotein, catalyzes the oxidation of a variety of primary aliphatic monoamines and diamines to their respective aldehydes and ammonia. This paper reports the construction and characterization of an enzyme electrode capable of detecting histamine and methylamine at +200 mV versus a saturated calomel reference electrode. The methylamine dehydrogenase isolated from Paracoccus denitrificans was used in conjunction with the insoluble mediator tetracyanoquinodimethane (TCNQ) to construct enzyme electrodes which will potentially provide simple rapid analysis of histamine without the need for the extensive sample pretreatments currently required in HPLC and GLC analysis. The linear response of this amperometric sensor, between 0 and 200 mu M, correlates well with elevated histamine levels predominant in patients with chronic myelogenous leukaemia, whilst the observed limit of detection, 4.8 mu M, compares favourably with the lower limits of detection reported for a potentiometric histamine sensitive enzyme electrode.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 1995. Vol. 10, no 07-jun, 569-576 p.
Keyword [en]
QUINOPROTEIN; TRYPTOPHAN TRYPTOPHYL QUINONE; TTQ; METHYLAMINE DEHYDROGENASE; ENZYME ELECTRODE; TETRACYANOQUINODIMETHANE; TCNQ; HISTAMINE
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65281ISI: A1995RH05200008OAI: oai:DiVA.org:liu-65281DiVA: diva2:394952
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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