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ONLINE MONITORING OF GLUCOSE, GLUTAMATE AND GLUTAMINE DURING MAMMALIAN-CELL CULTIVATIONS
GESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
GESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; .
GESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; .
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1995 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 10, no 07-jun, 543-551 p.Article in journal (Refereed) Published
Abstract [en]

Amperometric biosensors (based on rhodinised carbon electrodes) for glucose, glutamine and glutamate were constructed. The sensors were incorporated into a three cell parallel FIA system and used to monitor the three analytes on-line during two mammalian cell perfusion cultures. All measurements were made simultaneously from undiluted media sample. Use of the FIA system enabled easy and rapid exchange of the sensors, during cultivation. The inclusion of a calibration step, regularly for all sensors, helped to maintain the accuracy of all measurements. Comparison with off-line measurements indicated that all three biosensors operated successfully, providing accurate information.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 1995. Vol. 10, no 07-jun, 543-551 p.
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Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65280ISI: A1995RH05200006OAI: oai:DiVA.org:liu-65280DiVA: diva2:394954
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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TURNER, APF

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