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Ruthenized screen-printed choline oxidase-based biosensors for measurement of anticholinesterase activity
UNIV FLORENCE,DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT,I-50121 FLORENCE,ITALY; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; .
UNIV FLORENCE,DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT,I-50121 FLORENCE,ITALY; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; .
UNIV FLORENCE,DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT,I-50121 FLORENCE,ITALY; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
1995 (English)In: Mikrochimica Acta, ISSN 0026-3672, E-ISSN 1436-5073, Vol. 121, no 04-jan, 155-166 p.Article in journal (Refereed) Published
Abstract [en]

Plastic disposable choline biosensors based on ruthenized-carbon screen-printed electrodes were prepared and their use for monitoring organophosphorus pesticides and carbamates is described. The presence of 0.5% ruthenium on activated carbon mixed to form a simple graphite-based ink for the working electrode surface increased the sensitivity towards hydrogen peroxide. The choline biosensor is based on such an electrode coupled with choline oxidase immobilized by adsorption and was used to detect the inhibition effect of carbamates and organophosphorus pesticides on acetylcholinesterase. With the optimized procedure described (pH, buffer composition, incubation time, substrate concentration), concentrations of pesticides (Carbofuran) as low as 1 nM could be detected.

Place, publisher, year, edition, pages
Springer Science Business Media , 1995. Vol. 121, no 04-jan, 155-166 p.
Keyword [en]
choline sensor; amperometry; enzyme inhibition; thick film
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65273ISI: A1995TN60100012OAI: oai:DiVA.org:liu-65273DiVA: diva2:394960
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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Turner, APF

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