Development of a mass-producible glucose biosensor and flow-injection analysis system suitable for on-line monitoring during fermentations
1996 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 321, no 03-feb, 165-172 p.Article in journal (Refereed) Published
Screen printed amperometric glucose biosensors were constructed and optimised for use in a flow injection analysis (FIA) system. The sensors were fabricated using a catalytic metallised carbon based ink, allowing a working potential of +350 mV (Ag/AgCl). Overall the sensors displayed a high degree of reliability and robustness. A linear response was obtained over the range 0.1 to 25 mM glucose with a slope of 1.35 +/- 0.20 mu A/mM. The within electrode coefficient of variation at each concentration tested (n = 10) was less than 2% (or 0.1 mM for concentrations below 5 mM). In addition, it was demonstrated that a single sensor can be used in the FIA system over a seven day period indicating a high level of operational stability. These sensors fulfil the criteria necessary for a mass produced device, which would be suitable for the on-line monitoring of glucose consumption during microbial fermentation.
Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 1996. Vol. 321, no 03-feb, 165-172 p.
flow injection; biosensors; fermentation
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-65271ISI: A1996UA96100005OAI: oai:DiVA.org:liu-65271DiVA: diva2:394962