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A new approach for creating double-stranded DNA biosensors
RUSSIAN ACAD SCI,VA ENGELHARDT MOLEC BIOL INST,MOSCOW 117984,RUSSIA; RUSSIAN STATE MED UNIV,MOSCOW 117869,RUSSIA; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; .
RUSSIAN ACAD SCI,VA ENGELHARDT MOLEC BIOL INST,MOSCOW 117984,RUSSIA; RUSSIAN STATE MED UNIV,MOSCOW 117869,RUSSIA; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; .
RUSSIAN ACAD SCI,VA ENGELHARDT MOLEC BIOL INST,MOSCOW 117984,RUSSIA; RUSSIAN STATE MED UNIV,MOSCOW 117869,RUSSIA; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; .
RUSSIAN ACAD SCI,VA ENGELHARDT MOLEC BIOL INST,MOSCOW 117984,RUSSIA; RUSSIAN STATE MED UNIV,MOSCOW 117869,RUSSIA; CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; .
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1996 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 11, no 9, 903-911 p.Article in journal (Refereed) Published
Abstract [en]

The principle of sandwich-type biosensors based on liquid-crystalline dispersions formed from [DNA-polycation] complexes is outlined. These biosensors will find application in the determination of a range of compounds and physical factors that affect the ability of a given polycationic molecule to maintain intermolecular crosslinks between neighbouring DNA molecules. In the case of liquid-crystalline dispersions formed from [DNA-protamine] complexes the lowest concentration of hydrolytic enzyme (trypsin) detectable was approximate to-10(-14) M. (C) 1996 Elsevier Science Limited.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 1996. Vol. 11, no 9, 903-911 p.
Keyword [en]
biosensors; DNA; DNA liquid-crystalline dispersion; polycations; [DNA-polycation] complexes; intermolecular crosslinks; protamines; trypsin; CD spectra
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65268ISI: A1996UY83700009OAI: oai:DiVA.org:liu-65268DiVA: diva2:394966
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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