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Determination of anticholinesterase pesticides in real samples using a disposable biosensor
UNIV FLORENCE, DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT, I-50121 FLORENCE, ITALY; CRANFIELD UNIV, CRANFIELD BIOTECHNOL CTR, CRANFIELD MK43 0AL, BEDS, ENGLAND; ARPAT, DIPARTIMENTO PROV PISTOIA, PISTOIA, ITALY; .
UNIV FLORENCE, DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT, I-50121 FLORENCE, ITALY; CRANFIELD UNIV, CRANFIELD BIOTECHNOL CTR, CRANFIELD MK43 0AL, BEDS, ENGLAND; ARPAT, DIPARTIMENTO PROV PISTOIA, PISTOIA, ITALY; .
UNIV FLORENCE, DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT, I-50121 FLORENCE, ITALY; CRANFIELD UNIV, CRANFIELD BIOTECHNOL CTR, CRANFIELD MK43 0AL, BEDS, ENGLAND; ARPAT, DIPARTIMENTO PROV PISTOIA, PISTOIA, ITALY; .
UNIV FLORENCE, DIPARTIMENTO SANITA PUBBL EPIDEMIOL and CHIM ANALIT, I-50121 FLORENCE, ITALY; CRANFIELD UNIV, CRANFIELD BIOTECHNOL CTR, CRANFIELD MK43 0AL, BEDS, ENGLAND; ARPAT, DIPARTIMENTO PROV PISTOIA, PISTOIA, ITALY; .
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1997 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 337, no 3, 315-321 p.Article in journal (Refereed) Published
Abstract [en]

A choline amperometric biosensor based on screen-printed electrodes was assembled and used to assess the inhibitory effect of organophosphorus and carbamic pesticides on acetylcholinesterase activity both in standard solutions and real samples, Acetylcholinesterase catalyses the cleavage of acetylcholine to choline and acetate, therefore the amount of choline measured using the biosensor is directly related to the enzyme activity. The extent of enzyme inhibition can be used as an index of the amount of anticholinesterase pesticides present. The hydrophobicity of organophosphorus and carbamic pesticides led to the evaluation of organic, water miscible solvents for use in the proposed method. Berate buffer containing 1% v/v acetonitrile was selected since it exhibited the least influence on enzyme activity from the tested solvents (acetonitrile, acetone, tetrahydrofuran and ethylacetate). Other solvents (dimethylsulfoxide and methanol) were avoided as they exhibited electrochemical interferences. An inhibition calibration curve was obtained using carbofuran, a strong inhibitor of acetylcholinesterase. The lowest detectable standard solution (mean +/-3 standard deviation of the blank) was 2 mu gl(-1) following an incubation time of 10 min. The method was then applied to real samples (fruits and vegetables) showing its suitability as a rapid screening assay (12 min per test) for the assessment of anticholinesterase pesticides, The biosensor results were compared with a standard analytical technique (gas chromatography with nitrogen phosphorus detector, GC-NPD).

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 1997. Vol. 337, no 3, 315-321 p.
Keyword [en]
biosensors; enzymatic methods; pesticides; anticholinesterase pesticides
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65262ISI: A1997WG13400007OAI: oai:DiVA.org:liu-65262DiVA: diva2:394970
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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