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Monitoring of the glucose concentration during microbial fermentation using a novel mass-producible biosensor suitable for on-line use
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Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
1997 (English)In: Enzyme and microbial technology, ISSN 0141-0229, E-ISSN 1879-0909, Vol. 20, no 8, 590-596 p.Article in journal (Refereed) Published
Abstract [en]

A flow-injection analysis system was combined with a mass producible, disposable biosensor and was used to monitor glucose concentrations during several microbial fermentations. The biosensor was manufactured using thick film, screen printing technology. Unlike previous devices of this type, the biosensor was designed to make multiple measurements over extended periods rather than to operate as a one-shot sensor. One yeast, one lactic acid, and three E. coli bioreactor cultures were tested using either defined or complex media. Results from the sensor were compared with a standard spectrophotometric test kit. In samples containing glucose concentrations within the range of the biosensor and the test kit, good correlations were obtained between the two methods. In addition to glucose, microbial growth and pH were recorded. (C) 1997 by Elsevier Science Inc.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 1997. Vol. 20, no 8, 590-596 p.
Keyword [en]
biosensors; glucose; fermentation; flow-injection analysis (FIA); process monitoring
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-65257ISI: A1997XA98700005OAI: diva2:394975
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2013-10-04

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