liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Immunomagnetic separation with mediated flow injection analysis amperometric detection of viable Escherichia coli O157
University Florence, Dipartimento Sanita Pubbl Epidemiol and Chim Analit, Florence, Italy; .
University Florence, Dipartimento Sanita Pubbl Epidemiol and Chim Analit, Florence, Italy; .
University Florence, Dipartimento Sanita Pubbl Epidemiol and Chim Analit, Florence, Italy; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
1998 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 70, no 11, 2380-2386 p.Article in journal (Refereed) Published
Abstract [en]

The coupling of an immunological separation (using immunomagnetic beads) with amperometric now injection analysis detection of viable bacteria is presented. Using a solution containing Escherichia coli O157, the electrochemical response with two different mediators [potassium hexacyanoferrate(III) and 2,6-dichlorophenolindophenol] was evaluated in the FIA system. Antibody-derivatized Dynabeads were used to selectively separate E. coli O157 from a matrix The kinetics and the capacity parameters regarding the attachment of bacteria to the immunobeads were studied. The immunomagnetic separation was then used in conjunction with electrochemical detection to measure the concentration of viable bacteria. A calibration curve of colony-forming units (du) against electrochemical response was obtained. The detection limit for this rapid microbiological method was 10(5) cfu mL(-1), and the complete assay was performed in 2 h. Some advantages over ELISA methods are the direct detection of viable cells (and not total bacterial load) and the need for only one antibody (not enzyme-labeled), thus making the assay faster (only one washing step is necessary) and less expensive.

Place, publisher, year, edition, pages
ACS American Chemical Society , 1998. Vol. 70, no 11, 2380-2386 p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65251ISI: 000073981600032OAI: oai:DiVA.org:liu-65251DiVA: diva2:394983
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

Open Access in DiVA

No full text

Authority records BETA

Turner, APF

Search in DiVA

By author/editor
Turner, APF
In the same journal
Analytical Chemistry
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 128 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf