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Assessment of glucose oxidase behaviour in alcoholic solutions using disposable electrodes
Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
1998 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 368, no 3, 219-231 p.Article in journal (Refereed) Published
Abstract [en]

Solvent resistant screen-printed three-electrode devices were employed to assess the behaviour of free and immobilised glucose oxidase in water-miscible organic solvent/aqueous buffer mixtures. Three alcoholic solvents were examined, methanol, ethanol and isopropanol. A rhodinised-carbon electrocatalyst was employed to facilitate hydrogen peroxide oxidation at a decreased operating potential. The devices had a linear response range of 1-10 mU GOX and allowed detection of down to 2 x 10(-14) mol GOX. The measurement of 0-1 (v/v) glucose in 30% (v/v) ethanol was achieved in 2 min using these highly storage-stable enzyme electrodes. Such devices are likely to have widespread applications in the diagnostic and on-line monitoring fields where rapid analysis in alcoholic matrices is desirable. (C) 1998 Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 1998. Vol. 368, no 3, 219-231 p.
Keyword [en]
glucose oxidase; screen-printed electrodes; alcoholic solutions; organic phase; amperometric biosensors; glucose measurement
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65250ISI: 000074558000006OAI: oai:DiVA.org:liu-65250DiVA: diva2:394984
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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