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Immunosensor for 2,4-dichlorophenoxyacetic acid in aqueous organic solvent soil extracts
Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
1998 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 70, no 23, 5047-5053 p.Article in journal (Refereed) Published
Abstract [en]

The development of a simple electrochemical immunoassay procedure for the field-based quantification of the herbicide 2,4-D in methanolic soil extracts is presented. The sensor utilizes a competitive immunoassay format incorporating an immobilized antigen complex at the surface of a disposable screen-printed working electrode element, The extent of glucose oxidase-labeled antibody binding td the antigen-electrode is determined amperometrically and is related to sample analyte concentration. The performance of the sensor is assessed in buffer, 30% methanol, and methanolic soil extracts. The device is capable of quantifying 2,4-D in all three matrixes at the low ppm level with coefficient of variation values of 6.2-33.6%. The causes of the variation observed in the sensor response indifferent soil matrixes are examined and improvements proposed. The sensor, tested in parallel with a commercial 2,4-D immunoassay test kit, yields comparable quantitative data and detection limits while exhibiting greater assay simplicity.

Place, publisher, year, edition, pages
ACS American Chemical Society , 1998. Vol. 70, no 23, 5047-5053 p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65247ISI: 000077278700038OAI: oai:DiVA.org:liu-65247DiVA: diva2:394987
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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