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Application of dual-step potential on single screen-printed modified carbon paste electrodes for detection of amino acids and proteins
Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; University Calcutta, Department Polymer Science, Calcutta 700009, W Bengal, India; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
1999 (English)In: Fresenius' Journal of Analytical Chemistry, ISSN 0937-0633, E-ISSN 1432-1130, Vol. 364, no 02-jan, 154-159 p.Article in journal (Refereed) Published
Abstract [en]

A novel screen-printed biosensor for protein and amino acid (AA) estimation is reported. A rhodinised carbon paste working electrode (WE) was used in. the three-electrode configuration. Bromine was generated by the electrolysis of an acidic potassium bromide electrolyte on the working electrode when poised at an appropriate potential. The consumption of bromine by the protein and amino acid was then measured at a lower-potential. This sensor was used to detect most of the amino acids and some common proteins. The method is highly sensitive and can even detect L- and D- proline which give no response with enzyme electrodes. This sensor has also been used to detect protein/amino acid in fruit juice, milk, and urine. The unique feature of the sensor is that it does not require any reagent/chemical other than buffer. No surface treatment or modification of the WE is necessary and hence the sensor is stable for the lifetime of the basic screen-printed electrode, which is about a year. A very small amount of analyte (10 mu L) is required for direct detection. The sensor is inexpensive and disposable and may also be used for continuous-flow systems. This sensor may have useful application in process monitoring and control during the production of amino acids.

Place, publisher, year, edition, pages
SPRINGER VERLAG , 1999. Vol. 364, no 02-jan, 154-159 p.
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-65246ISI: 000080597100024OAI: diva2:394988
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2013-10-04

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