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Screen-printed amperometric biosensors for the rapid measurement of L- and D-amino acids
Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
1999 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 124, no 6, 865-870 p.Article in journal (Refereed) Published
Abstract [en]

Screen-printed three-electrode amperometric sensors incorporating L- and/or D-amino acid oxidase for the general purpose measurement of L- or D-amino acids is described. The working electrode incorporates rhodinised carbon, to facilitate hydrogen peroxide oxidation at a decreased operating potential, and immobilised enzyme. The devices responded to all 20 common L-amino acids and all of the D-amino acids examined, the exceptions being L- and D-proline. Linear response profiles were observed for L-leucine, L-glycine and L-phenylalanine with limits of detection of 0.47, 0.15 and 0.20 mM respectively. The devices were reproducible and exhibited stability over a 56 d test period. The biosensor compares favourably with a standard photometric amino acid test and was used to monitor milk ageing effects, The assay is cheap, simple to perform and rapid, requiring only buffer-electrolyte and a small sample volume.

Place, publisher, year, edition, pages
Royal Society of Chemistry , 1999. Vol. 124, no 6, 865-870 p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65245ISI: 000080940600009OAI: oai:DiVA.org:liu-65245DiVA: diva2:394990
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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