liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Chemical grafting of molecularly imprinted homopolymers to the surface of microplates. Application of artificial adrenergic receptor in enzyme-linked assay for beta-agonists determination
Cranfield University, Institute Biosci and Technology, Cranfield MK43 0AL, Beds, England; .
Cranfield University, Institute Biosci and Technology, Cranfield MK43 0AL, Beds, England; .
Cranfield University, Institute Biosci and Technology, Cranfield MK43 0AL, Beds, England; .
Cranfield University, Institute Biosci and Technology, Cranfield MK43 0AL, Beds, England; .
Show others and affiliations
2000 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 72, no 18, 4381-4385 p.Article in journal (Refereed) Published
Abstract [en]

A technique for coating of microplate weds with a molecularly imprinted polymer (MTP), specific for epinephrine, is presented. 3-Aminophenylboronic acid was polymerized in the presence of epinephrine using oxidation of the monomer by ammonium persulfate. This process resulted in the grafting of a thin polymer layer onto the polystyrene surface of the microplates. The polymer affinity was determined by an enzyme-linked assay using a conjugate of horseradish peroxidase and norepinephrine (HRP-N). It was found that imprinting resulted in increased affinity of the polymer toward HRP-N and epinephrine. Influence of the buffer pH and concentration on the polymer affinity was analyzed. It was shown that the MIP-coated microplates could be used for assay development and drug screening. The high stability of the polymers and good reproducibility of the measurements make MIP coating an attractive alternative to traditional antibodies or receptors, used in ELISA.

Place, publisher, year, edition, pages
ACS American Chemical Society , 2000. Vol. 72, no 18, 4381-4385 p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65235DOI: 10.1021/ac0002184ISI: 000089308300017OAI: oai:DiVA.org:liu-65235DiVA: diva2:395000
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Turner, APF

Search in DiVA

By author/editor
Turner, APF
In the same journal
Analytical Chemistry
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 142 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf