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Amperometric biosensor for formic acid in air
Natl Institute Working Life, Department Chemistry, S-90713 Umea, Sweden; Umea University, Department Publ Hlth and Clin Med, S-90581 Umea, Sweden; Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Natl Institute Working Life, Department Chemistry, S-90713 Umea, Sweden; Umea University, Department Publ Hlth and Clin Med, S-90581 Umea, Sweden; Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Natl Institute Working Life, Department Chemistry, S-90713 Umea, Sweden; Umea University, Department Publ Hlth and Clin Med, S-90581 Umea, Sweden; Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
Natl Institute Working Life, Department Chemistry, S-90713 Umea, Sweden; Umea University, Department Publ Hlth and Clin Med, S-90581 Umea, Sweden; Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
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2000 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 70, no 03-jan, 182-187 p.Article in journal (Refereed) Published
Abstract [en]

The possibility of developing a simple, inexpensive and specific personal passive "real-time" air sampler incorporating a biosensor for formic acid was investigated. The sensor is based on the enzymatic reaction between formic acid and formate dehydrogenase (FDH) with nicotinamide adenine dinucleotide (NAD(+)) as a co-factor and Meldolas blue as mediator. An effective way to immobilise the enzyme, co-factor and Meldolas blue on screen-printed, disposable, electrodes was found to be in a mixture of glycerol and phosphate buffer covered with a gas-permeable membrane. Steady-state current was reached after 4-15 min and the limit of detection was calculated to be below 1 mg/m(3). However, the response decreased by 50% after storage at -15 degreesC for 1 day. (C) 2000 Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2000. Vol. 70, no 03-jan, 182-187 p.
Keyword [en]
biosensor; air monitoring; formic acid; gas-permeable membrane; glycerol
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65232ISI: 000090126600026OAI: oai:DiVA.org:liu-65232DiVA: diva2:395002
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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