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A new reactive polymer suitable for covalent immobilisation and monitoring of primary amines
Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England; .
Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England; .
Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England; .
Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England; .
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2001 (English)In: Polymer, ISSN 0032-3861, E-ISSN 1873-2291, Vol. 42, no 8, 3603-3608 p.Article in journal (Refereed) Published
Abstract [en]

A new polymer capable of reacting with primary amines was synthesised from allyl mercaptan, o-phthalic dialdehyde and ethylene glycol dimethacrylate by radical polymerisation. Reactive hemithioacetal formed by allyl mercaptan and dialdehyde can bind primary amino groups without additional pre-activation forming the fluorescent isoindole complex. It gives a great opportunity to monitor binding and perform loading of the amino compounds onto the reactive surface. The reactive polymer is found to be an effective matrix for immobilisation of the proteins and other amino-containing compounds in affinity chromatography and could be used for their detection in solution. (C) 2001 Elsevier Science Ltd. All rights reserved.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2001. Vol. 42, no 8, 3603-3608 p.
Keyword [en]
amines; immobilisation; polymerisable hemithioacetal
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65227ISI: 000166619700030OAI: oai:DiVA.org:liu-65227DiVA: diva2:395007
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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