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Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays
Cranfield University, Institute BioScience and Technology, Silsoe MK45 4DT, Beds, England; .
Cranfield University, Institute BioScience and Technology, Silsoe MK45 4DT, Beds, England; .
Cranfield University, Institute BioScience and Technology, Silsoe MK45 4DT, Beds, England; .
Cranfield University, Institute BioScience and Technology, Silsoe MK45 4DT, Beds, England; .
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2001 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 16, no 12-sep, 701-707 p.Article in journal (Refereed) Published
Abstract [en]

A new technique for coating microtitre plates with molecularly imprinted polymers (MIP), specific for low-molecular weight analytes (epinephrine, atrazine) and proteins is presented. Oxidative polymerization was performed in the presence of template; monomers: 3-aminophenylboronic acid (APBA), 3-thiopheneboronic acid (TBA) and aniline were polymerized in water and the polymers were grafted onto the polystyrene surface of the microplates. It was found that this process results in the creation of synthetic materials with antibody-like binding properties. It was shown that the MIP-coated microplates are particularly useful for assay development. The high stability of the polymers and good reproducibility of the measurements make MIP coating an attractive alternative to conventional antibodies or receptors used in enzyme linked immunosorbent assay (ELISA). (C) 2001 Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2001. Vol. 16, no 12-sep, 701-707 p.
Keyword [en]
fluorescent immunoassay; ELISA; conjugated polymers; grafting on microplates; molecular imprinting
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Engineering and Technology
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URN: urn:nbn:se:liu:diva-65221ISI: 000172169500013OAI: oai:DiVA.org:liu-65221DiVA: diva2:395012
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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Turner, APF

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