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Development of an integrated capillary electrophoresis/sensor for L-ascorbic acid detection
University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Institute Biosci and Technology, Silsoe, Beds, England; .
University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Institute Biosci and Technology, Silsoe, Beds, England; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Institute Biosci and Technology, Silsoe, Beds, England; .
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2002 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 23, no 2, 209-214 p.Article in journal (Refereed) Published
Abstract [en]

A CE/biosensor for measuring ascorbic acid was developed by coupling a polyaniline optical sensor and capillary electrophoresis (CE). The capillary column was partially modified with a thin film of polyaniline redox sensitive material. Ascorbic acid was detected by monitoring the changes in optical absorbance occurring to the polyaniline film upon the reduction reaction. The sensor response (change in optical absorbance at 650 nm) is proportional to the concentration of ascorbic acid over a range of 2.5-250 mg/L and the response range has shown a clear dependence on the characteristics of the polymerized film. High specificity and sensitivity of the present method, low sample consumption, short times of response (ca. 2 min) and the reproducibility of the results demonstrate that the CE/polyaniline-sensor could be further employed in the study of the relation between the content of L-ascorbic acid in body fluids and clinical parameters, e.g., cell ageing.

Place, publisher, year, edition, pages
John Wiley andamp;amp; Sons, Ltd , 2002. Vol. 23, no 2, 209-214 p.
Keyword [en]
capillary electrophoresis; sensor; polyaniline; ascorbic acid; antioxidants
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65218ISI: 000173841000009OAI: oai:DiVA.org:liu-65218DiVA: diva2:395015
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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