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Repartition effect of aromatic polyaniline coatings on the separation of bioactive peptides in capillary electrophoresis
University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Institute Biosci and Technology, Silsoe, Beds, England; .
University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Institute Biosci and Technology, Silsoe, Beds, England; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Institute Biosci and Technology, Silsoe, Beds, England; .
2002 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 23, no 2, 203-208 p.Article in journal (Refereed) Published
Abstract [en]

The capillary walls of fused-silica capillary electrophoresis (CE) columns were modified with a thin film of polyaniline (PANI), providing open-tubular columns with a stable coating containing aromatic groups and amine functionalities. Fast and efficient separations were observed for small bioactive peptides under acidic conditions on PANI-coated columns. The mechanism of separation is based on hydrophobic interactions between the analytes and the polymeric matrix. Good reproducibility was observed from run-to-run. Due to the simple derivatization procedure, method flexibility, the uniformity of the coating and its stability, conjugated polymers could find practical application in capillary zone electrophoresis (CZE) separations.

Place, publisher, year, edition, pages
John Wiley andamp;amp; Sons, Ltd , 2002. Vol. 23, no 2, 203-208 p.
Keyword [en]
coating; capillary electrophoresis; polyaniline; peptides; electroosmotic flow; repartition effect
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65217ISI: 000173841000008OAI: oai:DiVA.org:liu-65217DiVA: diva2:395017
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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