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Surface imprinted beads for the recognition of human serum albumin
University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Cranfield Hlth, Bedford MK45 4DT, England; .
University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Cranfield Hlth, Bedford MK45 4DT, England; .
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Cranfield Hlth, Bedford MK45 4DT, England; .
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2007 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 22, no 10-sep, 2322-2328 p.Article in journal (Refereed) Published
Abstract [en]

The synthesis of poly-aminophenylboronic acid (ABPA) imprinted beads for the recognition of the protein human serum albumin (HSA) is reported. In order to create homogeneous recognition sites, covalent immobilisation of the template HSA was exploited. The resulting imprinted beads were selective for HSA. The indirect imprinting factor (IF) calculated from supernatant was 1.6 and the direct IF, evaluated from the protein recovered from the beads, was 1.9. The binding capacity was 1.4 mg/g, which is comparable to commercially available affinity materials. The specificity of the HSA recognition was evaluated with competitive experiments, indicating a molar ratio 4.5/1 of competitor was necessary to displace half of the bound HSA. The recognition and binding of the imprinted beads was also tested with a complex sample, human serum and targeted removal of HSA without a loss of the other protein components was demonstrated. The easy preparation protocol of derivatised beads and a good protein recognition properties make the approach an attractive solution to analytical and bio-analytical problems in the field of biotechnology. (c) 2007 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2007. Vol. 22, no 10-sep, 2322-2328 p.
Keyword [en]
molecularly imprinted polymers; human serum albumin; 3-aminophenylboronic acid; serum depletion; proteome
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65181DOI: 10.1016/j.bios.2006.12.034ISI: 000245541600072OAI: oai:DiVA.org:liu-65181DiVA: diva2:395055
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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P. F. Turner, Anthony

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