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Surface plasmon resonance imaging for affinity-based biosensors
University Firenze Polo Science, Dipartimento Chim, Sesto Fiorentino, FI, Italy.
University Firenze Polo Science, Dipartimento Chim, Sesto Fiorentino, FI, Italy.
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
University Firenze Polo Science, Dipartimento Chim, Sesto Fiorentino, FI, Italy.
2010 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 25, no 5, 957-966 p.Article, review/survey (Refereed) Published
Abstract [en]

SPR imaging (SPRi) is at the forefront of optical label-free and real-time detection. It offers the possibility of monitoring hundreds of biological interactions simultaneously and from the binding profiles, allows the estimation of the kinetic parameters of the interactions between the immobilised probes and the ligands in solution. We review the current state of development of SPRi technology and its application including commercially available SPRi instruments. Attention is also given to surface chemistries for biochip functionalisation and suitable approaches to improve sensitivity. (C) 2009 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2010. Vol. 25, no 5, 957-966 p.
Keyword [en]
Surface plasmon resonance imaging; SPRi; Affinity sensing; Immobilisation chemistry; Signal amplification immunosensor; Aptasensor; Optical sensor
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-65160DOI: 10.1016/j.bios.2009.08.039ISI: 000273945800001OAI: oai:DiVA.org:liu-65160DiVA: diva2:395065
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2017-12-11

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P. F. Turner, Anthony

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