Electrochemical Dissolution of Silver Nanoparticles and Its Application in Metalloimmunoassay
2010 (English)In: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 22, no 2, 191-198 p.Article in journal (Refereed) Published
This work demonstrates the use of silver nanoparticles as a simple electrochemical biolabel to induce 10(6) signal enhancement. We propose it mechanism of measuring the silver nanoparticles oil a specific screen-printed planar carbon electrode, without the requirement for the harsh oxidant or toxic reagents described in prior-art for gold sol methods. The possible measurement process was validated with orthogonal techniques such as UV/Vis spectroscopy. dynamic light scattering and by anodic stripping voltammetry (ASV). The findings Were utilized to develop a novel electrochemical sandwich immunoassay where the analyte concentration is directly proportional to ASV oxidation peak of silver. This technique in the future is envisaged to form the foundation of a generic Point of Care platform. The assay was applied to cardiac marker: myoglobin with detection limit of 3 ng/mL.
Place, publisher, year, edition, pages
John Wiley andamp;amp; Sons, Ltd , 2010. Vol. 22, no 2, 191-198 p.
Silver nanoparticles; Anodic stripping voltammetry; Electrochemical immunoassay; Nanoparticle dissolution; Myoglobin; Immunoassays; Voltammetry
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-65159DOI: 10.1002/elan.200900275ISI: 000274282100009OAI: oai:DiVA.org:liu-65159DiVA: diva2:395066